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Exempel

• Polyclonal antibodies were prepared against the period gene product, which influences biological rhythms in D. melanogaster, by using small synthetic peptides from the per sequence as immunogens.
• Certain gene functions, however, are inherently dosage-sensitive (Fisher and Scambler, 1994). These include: * gene products that are part of a quantitative signaling system whose function depends on partial or variable occupancy of a receptor, DNA-binding site, etc.; * gene products that compete with each other to determine a developmental or metabolic switch; * gene products that co-operate with each other in interactions with fixed stoichiometry (such as the α and β globins or many structural proteins). In each case the gene product is titrated against something else in the cell.
• In this case, we find that the majority of the predicted protein matches the FMR1 gene product but that we have introduced some novel peptide sequence at the amino-terminal end as well as some near the carboxy terminus.